Credit Units: 2
Lecturers: Mr. Ubak, N. G. (BSc, Biochemistry; M.Sc. Pharmacognosy), Email: firstname.lastname@example.org, College of Health Sciences, Dept of Pharmacy and Pharmaceutical Sciences
This course aimed to provide students with an understanding of biochemical concepts as a foundation for other subjects in the pharmaceutical sciences. This course gives an understanding of the molecular level of the chemical processes associated with living cells and how they affect human life in health and illness. The application of chemical laboratory methods for diagnosis, monitoring of treatment, and prevention of diseases is explained.
At the end of the course, students should be able to:
- explain the basic biochemistry of the body and biomolecules; the principal metabolic pathways and the basis of their regulation and interaction; the nature of information transfer and molecular biology methods; the underlying biochemical basis of cause, prevention and treatment of diseases.
- observe, measure biochemical compounds and make inference from biochemical information as related to adverse effects of drugs. Know the importance of enzymes and isoenzymes and their roles in assays and the diagnosis of diseases.
- understand metabolism and some common metabolic disorders.
- explain mammalian genetics and associated processes. Understand the basis of organ function tests and be able to carry out qualitative and quantitative analysis of biomolecules in body fluids.
- Introduction to biochemistry: Cell and its biochemical organization, transport process across the cell membranes. Energy rich compounds; ATP, UTP, GTP, CTP and their biological significance.
- Enzymes: Definition; Nomenclature; IUB classification; Factors affecting enzyme activity; Enzyme action; enzyme inhibition. Isoenzymes, and their assay and diagnostic applications; Coenzymes and their biochemical role and deficiency diseases.
- Carbohydrate metabolism: Glycolysis, Citric acid cycle (TCA cycle), HMP shunt, Glycogenolysis, gluconeogenesis, glycogenesis. Metabolic disorders of carbohydrate metabolism (diabetes mellitus and glycogen storage diseases); Glucose, Galactose tolerance test and their significance; hormonal regulation of carbohydrate metabolism.
- Fluid and electrolyte balance. Acid – base balance and pH of the internal environment. Water and buffers.
- Lipid metabolism: Oxidation of saturated, monounsaturated and polyunsaturated fatty acids; Ketogenesis and ketolysis; biosynthesis of fatty acids, metabolism of cholesterol; Hormonal regulation of lipid metabolism; Sphingolipids metabolism. Defective metabolism of lipids (Atheroslerosis, fatty liver, hypercholesterolaemia).
- Biological oxidation: Coenzyme system involved in biological oxidation. Electron transport chain (its mechanism in energy capture; regulation and inhibition); `Oxidative phosphorylation; Uncouplers of electron transport chain (ETC).
Methods of Instruction:
- Discussion groups
- Reading of articles from relevant scientific journals
Methods of Course Assessment:
. Evaluation will be based on the following criteria:
Class attendance 10
Semester’s Examination 70
Recommended Reference Materials:
- BERG, J. M., TYMOCZKO, J. L., STRYER, L. AND GATTO, G.J. (2015). Biochemistry. 8th Edn. New York: W. H. Freeman. ISBN: 9781464126109.
- LIEBERMAN, M. AND PEET, A. Marks’ (2017). Basic Medical Biochemistry: A Clinical Approach. 5th Edn. Lippincott Williams & Wilkins. ISBN: 9781496324818
- DEVLIN, T. M. (2010). Textbook of Biochemistry with Clinical Correlations. 7th Edn. John Wiley & Sons,. ISBN: 9780470281734
- CHATTERJEA, M. N. (2009). Textbook of Biochemistry for Dental, Nursing, Pharmacy Students. 3rd Edn.Jaypee Brothers Medical Publishers (P) Ltd,. ISBN: 978-8184485318
- TIMBERLAKE, K. C. (2011). Chemistry: An Introduction to General, Organic and Biological Chemistry. 11th Edn, Prentice Hall, ISBN: 9780321741042
Please click on the course content tab to have access to all required materials for this course.